Time-resolved fluorescence spectra of 7-hydroxycoumarin

Time-resolved fluorescence microscopy.

In fluorescence microscopy, the fluorescence emission can be characterised not only by intensity and position, but also by lifetime, polarization and wavelength. Fluorescence lifetime imaging (FLIM) can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, and time-resolved fluorescence anisotropy imaging (TR-FAIM) can measure the rotation...

Time-resolved fluorescence spectroscopy

Time Resolved Fluorescence of CdSe Nanocrystals

Theory for Time Resolved Emission Spectra

Explicit equations for the calculation of time resolved emission spectra from molecules undergoing collisional vibrational relaxation are presented. The equations describe the changes of the emission spectrum with time, after a delta puke excitation. Alternatively, they can be used to obtain the steady state (frequency resolved) emission spectrum as a function of the pressure of added buffer ga...

Time-Resolved Fluorescence in Photodynamic Therapy

Photodynamic therapy (PDT) has been used clinically for treating various diseases including malignant tumors. The main advantages of PDT over traditional cancer treatments are attributed to the localized effects of the photochemical reactions by selective illumination, which then generate reactive oxygen species and singlet oxygen molecules that lead to cell death. To date, overor under-treatme...